Final Preparation!

After confirming that the experimental trial worked, it is time to start. I plan to start off with two experimental sets, hence I will need 8 boxes, 4 boxes for each set. Each box is set up like the pre-experimental trial box, with approximately 280g of soil, 200ml soaked water, a little shelter made by the egg container and a food area shaped by a piece of filter paper (that way, there won't be any molds). I came in late this week to spray 15 times in #1-8 to moist the area in preparation when pill bugs come in next week. I will briefly explain again how #1-8 is used. In #1 and #5, there is the negative control group, female + female. In #2 and #6, there is the positive control group, male + female, which we can expect a second generation. Then #3 and #7 are the treatment 1 group, female + female with periodic interaction with males for once. Last but not least, #4 and #8 are the treatment 2 group, female + female with periodic interaction with males for twice. Once they all come in next week, I will sex them and then the cycle starts!

This is how my experimental set-up is...(cleaner than it usually is)

See the one experimental set on the left - the four piled up boxes.